In 1985 God, the Holy Spirit gave Evangelist Shirley K. Russell (a God- fearing woman and mathematics instructor at Morgan State University) a vision for a Christian fraternity and sorority. The development of this vision matured when Evangelist Russell shared the vision with two of her students, Doral R. Pulley and Joyce Mungo, who were very instrumental in organizing the "CALLING ALL CHRISTIANS" meeting on November 3, 1988. This is now known as Founder's Day.

The Lord moved on the hearts and minds of many of the attendees of the meeting to help in the manifestation of the vision of Alpha Nu Omega. The first fruits of Alpha Nu Omega consisted of 14 founding brothers and fifteen founding sisters. Collectively they labored along with the Visionary Founder Shirley K. Russell in establishing the Constitution of Alpha Nu Omega. This step enabled the organization to be incorporated on December 20, 1988, 47 days after the founding date. The National Headquarters was at this time established at Morgan State University in Baltimore, Maryland.

Many crucial decisions were made by the founders of Alpha Nu Omega, Inc. in regard to the administration of the organization. One decision called for the organization to operate as two distinct entities (a fraternity and a sorority), bound by a single constitution. At that time two executive boards were established. Founder Doral R. Pulley presided as the president of the fraternity, and Founder Joyce Mungo presided as the president of the sorority. Visionary Founder Shirley K. Russell remained the advisor to the brother/sister organizations. In October of 1989, the organization inducted its first line consisting of one brother and six sisters. The second line for the sisters was held in the fall of 1990. The sisters increased their membership by five.

In the Spring of 1991 the entire Alpha Nu Omega Family met and under the leading of the Lord decided to become a non-pledging organization. This decision led to the rapid addition of membership at the Alpha Chapter as well as a Beta Chapter in Fall 1991 and Gamma Chapter in Spring 1992. In the Summer of 1993 Alpha Nu Omega spread to the plains of Bible College at New Destiny Christian Center with the induction of Alpha-Alpha-I Chapter. College graduates and professionals have also latched onto the vision of Alpha Nu Omega with the establishment of the first graduate chapter, Baltimore/Washington Alumni Chapter in Baltimore, Maryland. The Lord allowed for the beginning of the reaping of Harvest 2000 with the induction of Delta Chapter in the Spring of 1997, Epsilon Chapter in the Fall of 1997, and Zeta Chapter in January 1998.

In the Fall of 1998, the National Executive Board voted to establish regions to manage the rapid growth of the organization. The regions established at that time were the Baltimore/Washington Region, the Delmar Region, and the Three Rivers Region. Various chapters have been established throughout those regions.

Change has taken place in Alpha Nu Omega on several occasions, but the brothers and sisters of Alpha Nu Omega Fraternity, Inc. and Alpha Nu Omega Sorority, Inc. hold to God's promise of membership growth and leadership in ministry due to spiritual growth which is found by walking in the Spirit and pressing toward the mark for the prize of the high calling of God in Christ Jesus (Philippians 3:14).